Electrical Stimulators for Physiological Research: Compliance

All stimulators incorporate a fundamental characteristic known as “compliance.” Compliance comes in two flavors, namely voltage compliance and current compliance. Voltage stimulators emulate a voltage source, up to a certain current compliance. Current stimulators emulate a current source,

By Alan Macy, BIOPAC Systems, Inc. 

All stimulators incorporate a fundamental characteristic known as “compliance.” Compliance comes in two flavors, namely voltage compliance and current compliance. Voltage stimulators emulate a voltage source, up to a certain current compliance. Current stimulators emulate a current source, up to a certain voltage compliance. The stimulator compliance limit is important information. Here are examples:

Current Compliance

A voltage stimulator is being used to establish a field stimulation in a tissue bath. The voltage pulse is 100 volts, with a width of 1 ms. The bath establishes a 1000 ohm impedance between stimulation electrodes. Therefore, 100 volts/1000 ohms is 100 ma. In this situation, the voltage stimulator must be able to provide a current compliance up to 100 ma for at least 1 ms. If the stimulator’s current compliance is limited to 50 ma, then the output voltage will be limited to 50 ma * 1000 ohms or 50 volts, even if the stimulator is being instructed to output 100 volts.

Voltage stimulators commonly operate with the help of high voltage DC-DC converters and storage capacitors to provide the needed compliance current. If the storage capacitors can’t store enough charge or are not sufficiently supplied, then voltage stimulators may exhibit a quality known as voltage droop. Voltage droop is the quality when the stimulator output voltage can’t be maintained over a specific time interval, due to lack of sufficient current compliance. If the above stimulator can only be current compliant to 100 ma for 0.5 ms, then the output voltage pulse will be 100 volts for 0.5 ms and then will decay in direct proportion to the current compliance limit.

Voltage Compliance 

A current stimulator is being used for neuromuscular stimulation between two small electrodes. The current pulse is 10 ma, with a width of 1 ms and there is a 10,000 ohm impedance between stimulation electrodes. Therefore, 10 ma * 10,000 ohms is 100 volts. In this situation, the current stimulator must be able to provide a voltage compliance up to 100 volts for at least 1 ms. If the stimulator’s voltage compliance is limited to 50 volts, then the output voltage will be limited to 50 volts / 10,000 ohms or 5 ma, even if the stimulator is being instructed to output 10 ma.

Current stimulators commonly operate with the help of high voltage DC-DC converters and storage capacitors to provide the needed compliance voltage. If the storage capacitors can’t store enough charge or are not sufficiently supplied, then current stimulators may exhibit a quality known as current droop. Current droop is the quality when the stimulator output current can’t be maintained over a specific time interval, due to lack of sufficient voltage compliance. If the above stimulator can only be voltage compliant to 100 volts for 0.5 ms, then the current pulse will be 10 ma for 0.5 ms and then will decay in direct proportion to the voltage compliance limit.

Example

In the case of transcranial Direct Current Stimulation (tDCS), a constant current of up to 2 ma (Is) may be applied through the cranium. Assuming a combined series electrode / skin junction resistance of 20 Kohms, a cranium resistance of 100 ohms and other loop series resistances considered negligible, then the total stimulation loop impedance (Zs) is 20.1 Kohms. Accordingly, in this situation, the voltage compliance (Vs) of the tDCS current stimulator must be at least:

Is * Zs = Vs
0.002 amp * 20,100 ohms = 40.2 volts

Given the variable nature of the combined series resistances in the stimulation loop, it’s generally helpful to double the expected compliance voltage requirement of the stimulator. Accordingly, in this case, a stimulator compliance voltage of 80 volts or higher is recommended. Because the series resistance in the loop is largely a function of the electrode / skin interface junctions, the choice of electrode type and contact area has a great effect on the voltage compliance requirements of the current stimulator. Other factors contributing to the voltage compliance requirement are the equilibrium potentials and over-potentials associated with the specified electrode / electrolyte / skin junctions. Furthermore, if high resistance electrode leads are used, the potential across the leads may become material.

 

BIOPAC Systems, Inc. provides life science researchers and educators with data acquisition and analysis systems that inspire people and enable greater discovery about life. BIOPAC lets you measure physiology anywhere, with innovative, compatible wireless, wearable solutions that can be used by anyone for meaningful discovery. We make high-quality scientific tools for physiology measurement and interpretation with superior compatibility and world-class customer service and support. Visit us at www.biopac.com.

Five Steps to a Better Physiology Lab – Preparation

Integrating new hardware and software into the Physiology lab curriculum…key points from instructors who have created successful labs that will greatly increase the chances of a rewarding lab experience for both students and instructor.

by William McMullen, BIOPAC Systems, Inc.

Integrating new hardware and software into the Physiology lab curriculum can be intimidating. Despite the best of early-summer intentions, in fall Instructors often find themselves apprehensively staring at a box full of unfamiliar equipment a few short days before the first lab. Luckily, the Biopac Student Lab excels in this situation, as it is easy to use and includes all materials necessary for students to prepare, record and analyze their data. This means that, in a crunch, if all one did was install the software and verify the hardware connections, most students should obtain meaningful data. Beyond the default setup, there are multiple customization options as described in the user support documentation. There are, however, some key points we have learned from instructors who have created successful labs that will greatly increase the chances of a rewarding lab experience for both students and instructor.

1. Organize the lab such that each student participates equally and each obtains recorded data from their bodies.
Students are more motivated when working with their own data. The Biopac Student Lab requires a minimum of three students for most lessons; a Recorder, a Director and a Subject. At the end of each lesson recordings, the students should rotate through these rolls and rerun the lesson until each student has recorded their own data. In order to provide enough recording time in a standard 2 hour lab period, the number of students per lab station should be four to five.

2. Setup basic Lesson Preferences before the first lab to minimize confusion.
Run each lesson* that will be used and setup the following:

  • If using lessons L02, L07-L09 or L12-L17, different models of transducers may be used. Check that the image and transducer descriptions in the lesson guide match the hardware used. If changes are needed, choose File > Lesson Preferences and select the desired transducer.
  • Disable undesired recording tasks by choosing File > Lesson Preferences > Lessons Recordings. If none of the recording tasks are desired (if using a custom procedure), disable all and only generic information will be displayed in the lesson guide.

*Note: If using the optional Curriculum Management System (available in BSL 4.1), only changes to the Host computer are required and all other computer/lab stations will be automatically updated. If not using this option, the changes must be made to each computer.

3. For Lessons L12 and L13 (Pulmonary Function), greatly reduce calibration time by matching the hardware with the software.
Label the lab station computer, the MP device and the Airflow Transducer (SS11LA/L) and make sure they are always matched. Run lesson 12 and check File > Preferences > Calibration Syringe Size to make sure it matches the syringe that will be used. Choose File > Preferences > Calibration Syringe Values and then select “Set once and use stored values”. Carefully run through the calibration using the syringe. From then on, when the students run the lesson, they will only need to calibrate for zero baseline (no syringe) which is easy and only takes a few seconds.

4. Students should have a basic understanding of the software before the first lab.
All Biopac Student Lab lessons use the same basic software tools and if students have a basic understanding of these tools in advance, they will experience greater success obtaining good data and having a positive lab experience. Students should be instructed to download the free Biopac Analysis software onto their personal computers.

This software not only provides the ability to analyze the data recorded in the lab but also allows students to prepare for a lab by studying the included tutorial videos and sample data. Some instructors give a test prior to the first lab to make sure students reviewed the BSL Tutorial Video.

5. Demonstrate proper electrode preparation and explain ways to minimize motion artifact. 
For lessons that use electrodes, good electrode contact and minimal EMG/motion artifact are keys to obtaining good data. Although this is explained in the procedures, it cannot be emphasized enough. Demonstrate the cleaning of the skin (with soap or alcohol) to remove dirt and oil and the abrading around the electrode site to remove the dead epidermal layers that impede electrical flow. Always place a drop of electrode gel on the electrode and wait at least 5 minutes after electrode placement before beginning a recording. To minimize EMG artifact, the student should be as relaxed and motionless as possible (unless procedure calls for movement). Talking, blinking (during EEG or EOG recording), laughing or deep breathing will add unwanted EMG artifact to the data.

These easily implemented steps will greatly assist students in obtaining good data from the very first lesson, leading to a positive lab experience and ultimately gaining a better understanding of Physiology.

 


BIOPAC Systems, Inc. provides life science researchers and educators with data acquisition and analysis systems that inspire people and enable greater discovery about life. BIOPAC lets you measure physiology anywhere, with innovative, compatible wireless, wearable solutions that can be used by anyone for meaningful discovery. We make high-quality scientific tools for physiology measurement and interpretation with superior compatibility and world-class customer service and support. Visit us at www.biopac.com.

Electrical Stimulators for Physiological Research

Highly-controlled electrical stimulation capability can provide a unique tool for the life science researcher to discern sensory function and associated physiological responses in living subjects. There are two types of electrical stimulators, voltage and current.

By Alan Macy, BIOPAC Systems, Inc.

Any physiological measurement is concerned with the subject of stimulus and response, because all physiological processes are subject to change with applied stimuli. The processes of life encapsulate the idea that environmental change precipitates sensory activity that results in stimulation. Because sensory activity is mediated by the flow of ions in the nervous system, highly controlled electrical stimulation capability can provide a unique tool for the life science researcher to discern sensory function and associated physiological responses in living subjects.

Computer-based systems can be used to define very exacting electrical stimuli and those signals can be directed to a subject via electrodes. The level of stimuli can be extremely wide and the shape of the stimuli can be finely controlled. Signals can be introduced directly into nerves or muscle fibers to evoke a specific response.

There are two types of electrical stimulators, voltage and current. A voltage stimulator behaves like a voltage source, in that it outputs a variable voltage with low output impedance. A current stimulator behaves like a current source, in that it outputs a variable current with high output impedance.

The primary physical expression that characterizes the behavior of electrical stimulators is:

Vs = Is * Zs

where:

Vs – stimulation voltage from stimulator
Is – stimulation current from stimulator
Zs – impedance of stimulation loop

Zs is the collective impedance of the complete stimulation loop. From a simplified view, Zs consists of the series combination of two electrode / skin junction impedances and the stimulated tissue volume. Zs has resistive and capacitive components, so the capacitive components charge in accordance to the expression:

Ics = Cs * (dVcs/dt)

where:

Ics – stimulation current through Cs
Cs – capacitive component associated with Zs
dVcs/dT – change in stimulus voltage, across Cs, as a function of time

Accordingly, electrical stimulation is associated with the charging of the stimulation loop capacitance. If fast electrical stimulation is required, then the stimulator should have high current level drive capability. For a given Cs, the speed of charging (dVcs/dt) will be higher (faster) as Ics is increased.

There are two major sub-impedances in the stimulation loop that define the portion of the stimulator output voltage (Vs) that is directly across the tissue volume (Vt):

Ze – electrode to site impedance (total of both sites)

Zt – stimulated tissue volume impedance

Vs = Is * Zs

Vs = Is * (Ze + Zt)

Vt = Vs * (Zt / (Ze + Zt))

Because of this relationship, the value of Vt might not be reliably specified, given a certain Vs.For any given tissue volume, electrical stimulation can be specified in terms of voltage across the tissue volume (Vt) or current through the tissue volume (It). In practice, because two terminal stimulation is simple to apply, current stimulation is often used because the current through the tissue volume (It) is the same as the sourced stimulation current (Is). However, if the stimulation current (It) is specified then Vt may be indeterminate. Sometimes, both (It) and (Vt) are required to be known, such as with bioimpedance measurements. In this case, voltage monitoring electrodes can be placed on the tissue volume so both (It) and (Vt) can be identified.

Compliance, an essential characteristic of electrical stimulators, is addressed in Electrical Stimulators for Physiological Research: Compliance.


BIOPAC Systems, Inc. provides life science researchers and educators with data acquisition and analysis systems that inspire people and enable greater discovery about life. BIOPAC lets you measure physiology anywhere, with innovative, compatible wireless, wearable solutions that can be used by anyone for meaningful discovery. We make high-quality scientific tools for physiology measurement and interpretation with superior compatibility and world-class customer service and support. Visit us at www.biopac.com.

BIOPAC life science data solutions recognized worldwide

Data acquisition and analysis for life science data

BIOPAC research and education systems are used in labs around the world and cited in thousands of publications. See how your colleagues are finding success with BIOPAC…

  • MP Research System user list

  • Biopac Student Lab education system user list

  • Peer-reviewed journal citations: use the citation search of your choice or see more than 2,700 BIOPAC citations on HighWire Press 

  • Scholarly references: search more than 19,800 BIOPAC references on Google Scholar

error: Content is protected !!