Back to the Wild: Measuring the Effects of Human Interaction with Nature

“I think that I cannot preserve my health and spirits, unless I spend four hours a day at least—and it is commonly more than that—sauntering through the woods and over the hills and fields, absolutely free from all worldly engagements.” —Henry David Thoreau

In his 1861 essay “Walking,” transcendentalist writer and philosopher Henry David Thoreau extolled the virtues, both physical and mental, of communing with nature. While the psychological and physiological benefits of human connection with the natural environment are well established, modern science has taken a closer look at these interactions. Today’s researchers often rely on interdisciplinary approaches that blend psychology, physiology, neuroscience, and environmental science to investigate the mechanisms behind nature’s restorative effects on mind and body. With continued urban growth and shrinking access to wild rural spaces, as well as the digitization of our daily lives, this field has become more relevant than ever.

Researchers study human interactions with nature to better understand the tangible health and psychological benefits associated with exposure to natural environments, such as reduced stress, improved mood, and enhanced cognitive function. Theories such as the Biophilia Hypothesis and Attention Restoration Theory (ART) suggest that humans have a built-in affinity for nature, which can support mental and emotional well-being. Psychophysiological research seeks to uncover the processes underlying these effects and to compare how different environments—whether urban, natural, or virtual—impact our bodies and minds.

The study of human-nature interaction encompasses a wide range of practical applications, including healthcare and mental wellness. Beyond health applications, this field informs decisions and policies regarding urban planning and architecture, such as the design of biophilic buildings and green spaces in cities. Research also aims to help individuals focus their attention, enhance creativity, and promote emotional well-being in both work and educational settings.

A study published in the Journal of Environmental Psychology examined how immersion in nature and exposure to nature-related images affect attention and could be used in attention restoration therapy and related applications. Researchers measured participants’ neural activity before, during, and after a four-day group camping trip in Utah using electroencephalography (EEG) to capture reward positivity (RewP), a brain signal associated with reward processing. During these periods, researchers measured how participants responded to extrinsic rewards, including monetary incentives. To ensure data integrity and minimize motion artifacts in the outdoor setting, the team utilized BIOPAC’s wireless BioNomadix EEG system and verified electrode contact using the EL-CHECK impedance checker. The study’s findings showed a significant decrease in neural responsiveness to extrinsic (monetary) rewards following immersion in nature. This suggests that increased time spent immersed in natural environments may recalibrate the brain’s reward sensitivity and promote intrinsic motivation. These findings have implications for a variety of behaviors related to extrinsic stimuli, such as the treatment of addiction.mountaintop

Not all research into human-nature interactions focuses on immersion in real-world environments. With the evolution of virtual reality (VR) technology, new research leverages artificial environments to elicit biophilic responses. A study by researchers at the Southwest Jiaotong University School of Architecture in China, published in Frontiers in Public Health, investigated how exposure to biophilic design elements affects stress recovery in older adults. A group of 25 participants over the age of 60 were exposed to stressors and then underwent sessions with randomized VR environments that included green walls and outdoor views. The researchers measured two key physiological signals: heart rate variability (HRV), which assesses parasympathetic activity, and skin conductance level (SCL), a marker of sympathetic arousal. Electrocardiogram (ECG) data were collected using a BIOPAC ECG amplifier, while electrodermal activity was gathered via an EDA amplifier. Both signals were recorded using a BIOPAC data acquisition and analysis system. Results found that participants exposed to environments with green features demonstrated significantly higher HRV and lower SCL, indicating enhanced autonomic recovery and reduced stress. These findings suggest that the biophilic benefits of nature exposure can be made available to individuals virtually when real-world immersion is not possible or available.

As these studies demonstrate, there exists a wide and varied frontier to explore how human interaction with nature shapes our physiological, neurological, and psychological well-being. For more information on the tools to assist with such research, visit our Webinars library for detailed video trainings on how to use these technologies in your nature-related study.


Whether your study participants are hitting the trail, climbing a mountain, or just relaxing in the garden, BIOPAC customer service reps are ready with the right solutions to take your next research project back to nature.

Aesthetically Pleasing: Exploring How the Brain Perceives the Arts

Beauty is in the eye of the beholder—a mostly subjective experience, so the saying implies. While we may share many common ideals about beauty and aesthetics, our reactions to art, music, and literature can diverge, converge, and overlap to a myriad of degrees. Neuroaesthetics explores how the brain perceives and responds to art, music, and other aesthetically evocative experiences. Combining neuroscience with psychology and the arts, researchers seek to understand why humans find certain images, sounds, or designs appealing and how these perceptions influence our emotions, cognition, and behavior. This interdisciplinary science has practical applications across a wide range of fields, including design, marketing, education, and mental health therapy, where understanding aesthetic experience can inform and enhance user engagement, learning, and emotional well-being.

Biometric signals provide insight into emotional and cognitive processing, allowing researchers to gain valuable data on how the brain creates and responds to aesthetically engaging stimuli. Commonly used techniques include electrodermal activity (EDA), which measures changes in skin conductance linked to emotional arousal; electroencephalography (EEG), which tracks brainwave patterns associated with attention and engagement; functional near-infrared spectroscopy (fNIRS), which monitors blood flow changes in the brain; and functional magnetic resonance imaging (fMRI), which provides high-resolution images of neural activity.

Additionally, the use of virtual reality (VR) and augmented reality (AR) is expanding in neuroaesthetics research, allowing scientists to create controlled, immersive environments that mimic real-world experiences. These technologies offer new ways to investigate how individuals engage with art and design in dynamic, interactive settings, pushing the boundaries of how we understand and harness aesthetic experiences.

Researchers in Shanghai, China, for example, investigated how different visual modes—2D and 3D VR—affect emotional arousal, demonstrating how immersive environments influence emotional experiences. The research team utilized EEG to monitor brain activity and EDA to measure skin conductance responses, both indicators of emotional arousal. EDA and EEG data were recorded while study participants wore VR headsets to view 2D and 3D environments. Data was collected and analyzed using a BIOPAC MP system with AcqKnowledge software. The findings revealed that 3D VR environments elicited more intense emotional stimulation than 2D settings, as evidenced by higher beta (21–30 Hz) EEG power and increased EDA responses. According to the published findings, results suggest that enhanced perception in 3D environments leads to greater emotional engagement, highlighting the potential of 3D VR in applications such as immersive therapy, virtual training, and interactive entertainment.

MusicA team of German researchers recently examined the intersection of neurology, psychology, music, and literature. Their findings, published in the journal NeuroImage, explored how melodic features influence the neural processing of spoken poems and songs, examining the interplay between language and music. Researchers employed fMRI to investigate the neural mechanisms underlying the processing of musical and nonmusical verse. The fMRI data revealed that both spoken poems and songs activated bilateral temporal auditory areas of the brain. One aspect of the study was the measurement of participants’ emotional responses to the stimulus, i.e., whether they liked the song or poem to which they were listening while the fMRI scan was conducted. To gather this data, participants interacted with a BIOPAC differential pressure transducer connected to the participants’ right and left index fingers to record the degree to which they enjoyed the music. The researchers stated the findings suggest that melodic properties are central to the aesthetic experience of both speech and music.

A study published by Britain’s Royal Society looked at the connection between aesthetic appreciation and emotional states, specifically how exposure to art or music can shift feelings of anxiety to curiosity. To understand how the brain perceives beauty, the researchers used EDA to assess participants’ physiological arousal during exposure to aesthetically pleasing stimuli, such as music. EDA data was gathered using a BIOPAC EDA amplifier connected to a data acquisition and analysis system while participants were administered anxiety-inducing electrical tactile stimulation during music listening sessions. The study demonstrated that aesthetic appreciation can reduce anxiety and promote curiosity by analyzing the EDA data alongside self-reported measures. The research team concluded that their results “might have potential applications across different human activities such as learning and therapy. The idea that aesthetic appreciation might affect anxious states and that expecting aesthetic rewards can transform anxiety into arousal and curiosity plays a central role in some psychotherapeutic models of human change.”

These examples provide a rough outline of a bigger picture, illustrating the many ways researchers use physiological signals to better understand the human mind’s response to art, music, and literature. For more information on the tools BIOPAC provides to assist in neurological and physiological research, see our online training and webinars.


Are you planning a research project to investigate the intersection of aesthetics and neuroscience? Contact a BIOPAC representative in your area to identify the right resources and equipment for a successful study.

Only the Lonely: The Human Impact of Social Isolation

Humans by their nature are social creatures. A sense of community, daily interactions, and shared experiences are tied to our overall mental and physical well-being. Few events in modern history have shut down such interactions more thoroughly than the outbreak of COVID-19, which forced millions into prolonged isolation. As lockdowns and social distancing became the norm, an increasing number of people reported heightened feelings of loneliness, anxiety, and depression. Beyond the pandemic, other modern social factors contribute to isolation, including the growing reliance on social media and virtual interactions in place of face-to-face connections. While technology offers new ways to stay in touch, excessive screen time and digital communication can sometimes deepen feelings of loneliness by reducing meaningful, in-person social engagement. In the wake of these societal shifts, researchers are working to understand the long-term consequences of social isolation on both mental and physical health.

Studies suggest that loneliness isn’t just an emotional experience—it has measurable physiological effects on the body. Using advanced biometric signals, researchers are trying to uncover how isolation influences our nervous system, stress responses, and overall wellness. Heart rate variability (HRV) provides insights into autonomic nervous system balance, while electrodermal activity (EDA) reflects changes in stress and emotional arousal. Respiration patterns, electromyography (EMG) readings, and other physiological markers help paint a complete picture of how isolation affects both mind and body. By combining psychological assessments with physiological data, scientists aim to better understand how chronic loneliness can contribute to conditions such as cardiovascular disease, weakened immune function, and mental health disorders.

In one such study published in Britain’s Royal Society Open Science journal in 2024, researchers looked at the effect of social isolation on human adolescents, specifically their ability to learn to perceive threats. Study participants between the ages of 16 and 19 underwent sessions of complete isolation and isolation combined with virtual social interaction, as well as baseline sessions. Following each session, study participants reported their psychological state and completed a threat learning task (participants learned to associate neutral shapes with an aversive sound) to measure physiological response to learned threats and safety cues. During these sessions, researchers recorded participants’ EDA signals using BIOPAC EDA finger transducers connected to an MP36R data acquisition and analysis system running AcqKnowledge software. Results showed heightened EDA levels during threat extinction following isolation compared to the baseline. Researchers reported that their findings suggest that isolation and loneliness among adolescents might increase vulnerability and increase the instances of emotional disorders such as phobias, anxiety, and obsessive-compulsive disorder (OCD).

A team of researchers in Brazil compared how giving and receiving of social touch as well as heart rate variability serve as accurate predictors of loneliness in college-age adults. Participant loneliness levels were measured using the UCLA Loneliness Scale. Participants completed a psychiatric screening questionnaire that reported loneliness, social support, and social touch levels. Participant HRV measurements were taken via electrocardiographic (ECG) signal processing using a BIOPAC ECG amplifier connected to a 16-channel MP data acquisition and analysis system running AcqKnowledge. According to the researchers’ published findings, “social support and social touch, specifically receiving touch, accounted for a significant proportion of the variance in loneliness. However, neither giving touch nor resting HRV was a significant predictor of loneliness.”alone

A study by researchers from the University of Bonn examined the link between loneliness and how males and females process trauma and react to fear-inducing stimuli. Over three days, participants were exposed to experimental trauma and evoked intrusive thoughts. Researchers employed functional magnetic resonance imaging (fMRI) scans to assess neural habituation to fearful faces and fear learning (conditioning and extinction) before trauma exposure. Fear conditioning and extinction tasks used a BIOPAC MP system to deliver stimulus in the form of electric impulses. EDA and respiration data were recorded using the BIOPAC data acquisition and analysis system. Study results revealed “a significant interaction between loneliness and sex such that loneliness is associated with more intrusions in men, but not in women.” The implication is, according to the study’s authors, “that loneliness may confer vulnerability to intrusive memories after trauma exposure in healthy men and that this phenotype relates to altered limbic processing of fear signals.”

As the physiological and psychological effects of the COVID-19 pandemic continue to resonate and we grapple with the isolating influence of technology, increasing numbers of research studies are being launched to measure these impacts. Studies into human isolation and loneliness represent just one small corner of a broad spectrum of research on the topic. For information on how BIOPAC equipment is being used in such research areas, check out our on-demand webinars for additional ideas on how to deploy BIOPAC resources in your next study.


Are you planning a research project investigating the effects of isolation, loneliness, or other related subjects? Reach out to our team of experts to help you get started with the right resources and equipment.

 

 

 

Facing Fear: Measuring What Scares Us

In many countries, October ushers in the season of ghosts, goblins, and all manner of frightening things that inhabit our deepest fears. While the popularity of Halloween reflects the thrills many derive from a good scare, facing one’s fears is not always fun and games. Our fears can protect us from danger, but they can also hamper individuals from performing the simplest tasks and negatively impact one’s quality of life and mental health.

The study of fear conditioning and fear learning sheds light on how humans and animals develop responses to threatening stimuli. Research often relies on psychophysiological measurements—techniques that capture the body’s physical responses to fear. Key among these measurements are skin conductance response (SCR), electrodermal activity (EDA), heart rate variability (HRV), respiration, and electromyography (EMG).

SCR and EDA track changes in sweat gland activity, providing a direct measure of emotional arousal when a person experiences fear. HRV and respiration rate reveal the autonomic nervous system’s reaction to stress, indicating shifts between sympathetic (fight-or-flight) and parasympathetic (rest-and-digest) states. EMG measures muscle activity, especially facial muscles, to detect subtle expressions or physical tension associated with fear. Understanding these mechanisms has practical applications in understanding and treating anxiety disorders, phobias, and post-traumatic stress disorder (PTSD). By analyzing the body’s automatic fear responses, scientists can develop targeted therapies to help individuals recondition or dampen these reactions.

A study published in the Journal of Behavior Therapy and Experimental Psychiatry used a range of indices to assess learned fear (unconditioned stimulus expectancy or US-expectancy) in individuals. These included both self-reporting and measurements of autonomic response such as skin conductance. Researchers using E-Prime Stimulus Presentation Systems, paired various neutral visual stimuli with unconditioned stimuli in the form of electrical shocks delivered via a constant voltage stimulator coupled with a BIOPAC STM100C amplifier. After a brief break, both neutral stimuli were administered without shock. Skin conductance response data was gathered and analyzed during each phase with a BIOPAC data acquisition and analysis system and software. Results showed a positive correlation between US-expectancy ratings and skin conductance response.

facing fearResearchers at the University of Bologna in Italy took a different approach to measuring the psychophysiological mechanisms of fear learning by focusing on HRV. In their study, published in the journal Psychophysiology, they explained that “understanding transient dynamics of the autonomic nervous system during fear learning remains a critical step to translate basic research into treatment of fear-related disorders.” Researchers gathered SCR and electrocardiogram (ECG) data. As with the previous study, mild electrical shocks were applied as unconditioned stimuli and paired with neutral or conditioned stimuli (CS). SCR signals were acquired using a skin conductance transducer attached to the participants’ fingertips connected to a BIOPAC EDA amplifier. ECG data was collected using disposable surface electrodes connected to an ECG100C amplifier. EDA and ECG signals were processed with a BIOPAC data acquisition and analysis system running AcqKnowledge software, which calculated HRV from the ECG data. Researchers concluded that “learned fear elicited a transient heart rate deceleration in anticipation of noxious stimuli.” They added that the study’s findings “provide a proximal measure of the involvement of cardiac vagal dynamics into the psychophysiology of fear learning and extinction, thus offering new insights for the characterization of fear in mental health and illness.”

Another study published in the journal Psychophysiology examines fear learning by applying a broad range of physiological signals and responses to determine which provides the “optimal measurement of human trace fear conditioning.” For this study, researchers compared SCR, pupil size response, heart period response (via ECG), respiration amplitude response, and electromyography (EMG) to measure participants’ responses to electrical current, audio, and visual stimulation. EDA data was gathered via a BIOPAC EDA amplifier, while ECG, EMG, and respiration data were fed to BIOPAC ECG100C, EMG100C, and RSP100C amplifiers, respectively. All data was processed using BIOPAC data acquisition and analysis systems and software. Results of the experiment suggested that startle eye-blink response through EMG provided the most sensitive metric for measuring fear conditioning.

Fear is just one of the emotions that can be studied through the body’s affective response to stimuli. Read more about the relationship between affect and emotion or watch our webinar on Emotion and Objective Physiological Measures.


Are you facing the reality of putting together your own fear-based study? Don’t be afraid to contact your local BIOPAC customer service representative for help finding the right tools for the job.

4T|Phys 2023: BIOPAC Shares Tools, Trends, Techniques & Technology at UCSB

They hailed from across the globe, from Arkansas to Zanzibar and all points between. Students, teachers, researchers, and innovators gathered on the campus of UC Santa Barbara, overlooking the Pacific Ocean. They came to learn, share, and explore breakthroughs and best practices in physiology and related fields. 4T|Phys kicked off July 17 under clear California summer skies, an idyllic setting for discovering the Tools, Trends, Techniques, and Technology driving physiology education and exploration.

4T|Phys 2023 was the third installment in BIOPAC’s highly successful series of biennial industry conferences that began in 2017. After a two-year hiatus during the global Covid-19 pandemic, 4T came roaring back this summer with more workshops, more tracks, more sponsors, more presenters, and more countries represented than ever before. Over the course of three days, nearly 100 attendees participated in hands-on sessions with some of the top minds in physiology research, experiencing the latest tools for gathering and analyzing data.

BIOPAC’s in-house experts were joined by an array of authorities from the fields of physiology, psychology, imaging (fNIRS and fMRI), neuroscience, cardiovascular research, eye tracking, virtual reality (VR), biology, and engineering. Participants took part in workshop schedules drawn from five separate tracks that included fundamentals, which focused on the basics of data acquisition and analysis. The research track took attendees on a deeper dive into techniques and technologies, including recording evoked potentials, screen-based eye tracking, impedance cardiography using trans-radial bioimpedance velocimetry (TREV), stimulus presentation, exercise physiology, and many others. Participants were also able to tour UC Santa Barbara’s state-of-the-art Brain Imaging Lab to see up close how BIOPAC products are being used in both functional MRI (fMRI) and TREV studies.

“It was an incredible experience that left a lasting impact on me by helping me to understand more about physiological data collection and analysis,” said Dr. Parth Trivedi, a 4T|Phys attendee. “The choice of presenters and the topics covered were truly inspiring, offering valuable knowledge and fresh perspectives that enriched my understanding.”

4T|Phys 2023 Attendee MapThis year’s conference featured several new tracks such as a dedicated VR track led by partner company WorldViz. The WorldViz team showcased virtual reality solutions in a series of workshops that introduced how VR is being integrated in physiology and psychology research, explained how to create effective and immersive VR experiments, and demonstrated their latest PRISM technology for creating fully immersive projected 3D virtual environments.

Several other partner companies introduced their technologies and products both in the 4T exhibition hall and in workshop sessions. Argus Science presented their ETVision eye-tracking glasses that synch seamlessly with AcqKnowledge. Cognixion demonstrated the Cognixion One Axon-R—Research Edition wearable BCI-AR computing platform, which is being used to help individuals with neurodegenerative conditions and severe motor impairments communicate and interact with their environments assisted by augmented reality. Seenel Imaging showed off their MedelOpt Systems for whole-head EEG with fNIRS, including new VR integration. Participants also got a chance to try on the Research Ring by Senstream, a new user-friendly wearable research ring, which allows easy recording of EDA, PPG, Temperature, and ECG signals. CNSystems showed off the company’s NIBP continuous noninvasive blood pressure (NIBP) and hemodynamics devices.

If you missed the educational and networking opportunities at 4T|Phys 2023, don’t despair. We will be back again in a few years, bigger and better than ever. Watch this space for updates on our next installment. In the meantime, see our Video Tutorials and Webinars On Demand for trainings and seminars on our products and partners.


Make sure you never miss a BIOPAC event and keep up with the latest Tools, Trends, Techniques, and Technologies by contacting your local BIOPAC sales representative.

Effort Rewarded: Tools for Measuring Motivation

A job well done may be its own reward, but the mechanisms that drive humans to produce better results are far more complex. Much research has been devoted to the study of motivation and the psychophysiological processes that drive our behavior in pursuit of a goal, whether in relationships, on the job, or in education.

The nature of motivation is controversial, and a variety of competing theories have been put forward regarding what a motivational state comprises. Some researchers have argued that motivation is driven by specific human needs, like food, safety, or respect from one’s peers. Motivation may be categorized as intrinsic, meaning an individual pursues an activity because the activity itself is enjoyable or interesting, or extrinsic, which is when the pursuit offers some external reward.

Research into motivation has drawn interest across a wide range of fields, particularly in the areas of business and education. In the realm of business, corporations are especially focused on the motivation to work, which impacts productivity. In an era where buzzwords such as “quiet quitting” and “bare minimum Mondays” have entered the lexicon, companies are keen to know what motivates workers. Similarly, educators are looking to what types of intrinsic and extrinsic motivational factors are most effective in helping students achieve their learning goals. A key metric for measuring motivation is effort, which can be measured with a range of tools readily available to researchers.

work harderA 2020 Harvard University study published by the American Psychological Association investigated the differences in how adolescents and adults translated motivation into action based on the physical effort exerted for financial reward. Participants age 12 to 23 exerted force by squeezing a BIOPAC hand dynamometer in response to visual cues indicating monetary rewards from $.05 to $.75. The dynamometer, connected to a DA100C amplifier, measured grip force, which the participant saw displayed on a screen as a vertical bar. These tools provided the research team with a means of accurately demonstrating quantitative variations in motivational behavior across various age groups in pursuit of effort-based rewards.

A similar approach was used in a 2019 study from the Netherlands that examined eating behavior by “quantifying the dynamic aspects of appetitive motivation.” As with the above example, researchers relied on a BIOPAC device to measure grip effort, in this case, a bulb transducer connected to an MP36 data acquisition system running Biopac Student Lab software. By squeezing the bulb, participants were able to self-administer sips of chocolate milk via a tube in response to stimuli while experiencing various states of hunger and satiation. The process developed for this study, termed the “Grab-to-Eat Task”, or GET, successfully showed that handgrip effort provided an accurate metric for “evaluating motivational aspects of food consumption” with implications for further research into eating behavior and weight control.

Techniques for measuring motivation are not limited to grip effort. A 2019 study published by the National Institute of Health relied on cardiovascular signals to measure the willingness of older adults to engage in cognitively demanding tasks. Blood pressure and heart rate data were collected using a CNAP monitor connected to a BIOPAC MP150 system, which provided a detailed measure of effort exerted through the participants’ physiological response to each task.

These examples just begin to touch on the ways various psychophysiological measures are being applied to the study of motivation and effort. For additional insight, view the BIOPAC webinar on tools for measuring effort and motivation in the lab.


For more information on how to gather and integrate effort-related data into your motivation study, contact your regional BIOPAC sales representative.

Eye-Catching: Mobile Tech Enhances Immersive Experiments

Freed from the limitations of stationary eye tracking technology, mobile eye tracking enables researchers to study their subjects while they perform a wide range of immersive tasks in natural settings.

Sixteenth-century pediatrician Thomas Phaer mused that “the eyes are the windows to the soul.” These sensory organs and their movements certainly provide a wealth of information to researchers studying the interaction of mind and body. Eye tracking has long been a key tool of physiological and psychophysiological research, and recent developments in mobile eye tracking take this valuable data resource out of the confines of the lab and into the real world.

Freed from the limitations of stationary eye tracking technology, mobile eye tracking enables researchers to study participants while they perform a wide range of immersive tasks in natural settings. These self-contained devices come in a variety of form factors including wearable glasses that provide untethered eye tracking data acquisition in real time that can be combined with other physiological data collected during live experiments.

Today’s lightweight mobile devices can record a broad spectrum of eye tracking data, including pupil size and position, gaze path, vergence angle, and areas of interest (AOI). The latter connects stimuli to eye-movement metrics, providing data on the participant’s psychophysiological response to stimuli within their field of vision. In previous blog posts and webinars, we have explored how mobile eye tracking technology gathers this data. Numerous studies have been published that demonstrate its potential in physiological and psychophysiological research. Settings and applications include user experience, sports and physical therapy, public safety and law enforcement, driving and equipment operation, and work environments from the office to the factory floor.

Mobile eye tracking technology allowed researchers at The University of Sheffield to take their experiments to the city streets for a 2020 study into how different lighting affects cyclists navigating urban environments at night. Mobile eye tracking provided data on the gaze behavior of nocturnal cyclists in parallel with skin conductance response (SCR) to better understand how obstacle detection is influenced by variations in type, location, and level of lighting. Bicycles and their riders were equipped with a BIOPAC data acquisition unit and audio stimulation units along with a BioNomadix EDA transmitter and electrodes to record the participants’ SCR. Eye tracking data, including AOI, were synchronized with SCR data and analyzed using AcqKnowledge software to determine how the riders reacted to a variety of lighting conditions. Researchers concluded that lighting plays a significant role in riders’ comfort levels at night and that improved lighting could increase urban bicycle use.

VGM playerLess intrusive by design, mobile eye tracking can provide indoor experiments with a heightened degree of immersion. A study at the University of British Columbia used mobile eye tracking glasses to create a more natural environment to investigate how the immersive effects of electronic gaming machines (EGM) impact gambling addiction. The study looked at how machine design triggered psychophysiological responses in participants by assigning various AOI to EGM screens. ECG data were recorded along with respiration effort via a BIOPAC respiration effort transducer and amplifier, which were fed to a data acquisition unit and synchronized with AOI data recorded via mobile eye tracking glasses. The study was able to successfully identify several key markers for game immersion using AOI that suggest behavioral links between immersion and gambling addiction.

Possible applications for mobile eye tracking in research continue to expand with the evolution of the technology. To learn more about recent developments in mobile eye tracking, check out our latest webinar on mobile eye tracking glasses with AcqKnowledge integration.


Learn more about how to integrate mobile eye tracking into your next research project by contacting your local BIOPAC sales representative.

Guilt Trips: Taking Measure of Our Moral Compass

“So full of artless jealousy is guilt, It spills itself in fearing to be spilt”
—Gertrude, William Shakespeare’s “Hamlet”

In Act 4 of Shakespeare’s famous tragedy, Hamlet’s mother Gertrude points out that the harder one tries to conceal guilt, the easier it is to expose. Little does she realize that she may have hit upon how future researchers approach the study of guilt in the lab. For practical purposes, it is a primary motivator in moral action and impacts how an individual responds to certain stimuli.

Psychologists have developed a myriad of tests to gauge guilt in a clinical setting such as the Test of Self-Conscious Affect (TOSCA), a multiple-choice test that tracks responses to various moral scenarios. While such techniques have been useful in linking guilt with conditions such as depression and anxiety, they have also shown their limitations in providing a deeper understanding of guilt’s influence on behavior, development, and psychological health.

In a recent BIOPAC webinar, UCSB professor Dr. Hongbo Yu addressed these limitations. Scenario-based tasks, he points out, are non-committal in examining the causes of guilt and do a poor job of measuring behavioral tendencies linked to guilt. Interaction-based research methods, however, allow participants to experience emotions in real-time, allow direct control of the causes of guilt in the lab, and allow for direct measurement of responses to stimuli.

As discussed in prior posts on affect, the body’s physiological manifestation of emotion, there is a range of methods and physiological signals available to researchers for measuring affect and gaining a better understanding of emotional states like guilt. Dr. Yu utilized eye-tracking, electrodermal activity (EDA), and functional magnetic resonance imaging (fMRI) in his examples. These are just a few of the signals currently being used in the lab to measure guilt.

One of the practical applications of guilt research is detecting when a subject is concealing the truth, as one would with a polygraph test. A recent study conducted by researchers at the University of Wisconsin-Madison’s Department of Psychology sought to gauge deception among research participants taking the Concealed Information Test (CIT). The goal was to improve upon traditional polygraph test signals such as heart rate and respiratory rate. Respiration data was gathered using a respiration transducer belt while ECG signals were collected with BIOPAC electrodes. In addition, researchers gathered EDA data using a transducer and finger electrodes. The study found that thGuilty Girle EDA signal was significantly more effective in accurately measuring physiological responses during the test procedure.

A 2018 study by researchers at Liverpool Hope University looked at the link between guilt and aggression in children by examining activity in the autonomic nervous system during so-called “bad behavior.” The study measured respiratory sinus arrhythmia (RSA) and skin conductance in children 4–8 years old while they imagined engaging in antisocial activities. EDA and ECG signals were gathered via wireless amplifiers and fed to an MP150 data acquisition unit (superseded by the MP160) running AcqKnowledge software. The study found a linkage between physiological responses to aggression and guilt that may provide a better understanding of the mechanisms behind guilt and related behavior in early and middle childhood.

These are just a few examples of how physiological signals can be applied to measuring guilt in research. BIOPAC offers a wide range of data acquisition, stimulus presentation, and VR options suitable for creating custom guilt research environments in the lab.


For additional information, contact your BIOPAC representative to learn more about the range of signals that can be utilized to gather data on guilt and other emotional states in the lab.

Measuring Moods: Signals for Insight into Emotion

“Do you ever look at someone and wonder what is going on inside their head?”

Joy, an anthropomorphized manifestation of happiness, delivers this line at the opening of Pixar’s 2015 animated hit “Inside Out,” a film that explores the emotional struggles of its young protagonist. She could have been speaking for generations of researchers who have plumbed the depths of the human psyche for a better understanding of the mechanisms behind our feelings, moods, and emotions.

When today’s researchers talk about emotion, they typically do so in terms of affect, the underlying physiological and psychological experience of feelings and emotions as a reaction to stimuli. Affect and emotion are not the same thing, per se, but are fundamentally connected. While the feelings we describe as fear, joy, love, hate, etc. remain abstract, affect is more concrete. BIOPAC co-founder and head of R&D describes it as “the measurable quality or manifestation of emotion in the human body.”

This manifestation can be measured in the human nervous system’s response to stimuli. Depending on the nature of the stimuli and how this sensory data is interpreted by the central nervous system, an array of physiological responses can take place: heart rate changes, the stomach and muscle groups tense, skin temperature responds, triggering sweat glands, among others.

The signals corresponding to these reactions provide researchers with valuable data to help them better understand affect and emotion. These signals—electroencephalogram (EEG), electrodermal activity (EDA), electromyogram (EMG), heart rate, and respiratory rate, to name a few—can be recorded and measured using a range of currently available sensing technologies.

As an example, a 2019 study published through the Queensland University of Technology used respiration signals as a means of classification for human emotions such as happiness, sadness, surprise, disgust, fear, and anger. In the study, breathing signals, including airflow rate and volume, were acquired and recorded using a BIOPAC MP36 data acquisition unit while participants were shown seven film clips, each associated with a different emotion. The study concluded that the respiratory data was 80% effective in accurately classifying affective responses to the film stimuli.

A team of researchers at the Khwaja Fareed University of Engineering and Information Technology in Pakistan took their investigation into the connection between physiological signals and emotion a step further. They used a similar approach by measuring participant response to movie clips, however this time, four types of physiological signals were acquired, including ECG, EMG, respiration, and skin conductivity (SC). The data was acquired with a wide range of signal sensors and analyzed via a BIOPAC MP160 DAQ System.Signals for Emotion

Signal sensing technology also allows researchers to study emotion in multiple participants simultaneously, as was the case in a 2020 dyadic study of how a parent’s stress and emotional suppression affects their children. The study measured physiological responses of both parents and children using ECG and impedance cardiography (ICG) via a BIOPAC MP150 data acquisition unit.

The mysteries of human emotion are being unlocked through a growing understanding of the affective response to stimuli. For more information on how physiological signals can be gathered and analyzed for this purpose, see our app note on emotional state physiology as well as our webinar Emotion and Objective Physiological Measures.


Contact BIOPAC for the latest information on how we can help you measure affect in the lab.

‘Tis the Season for Stress: Research Tools for Anxious Times

Holiday-related stress is as much of a seasonal tradition as family gatherings, frantic shopping forays, and over-indulgence in food and drink. Add to this potent mixture nearly two years of pandemic-induced tension and we have a volatile cocktail of emotions and anxiety awaiting us in November and December. What better time to study the causes and effects of stress?

Research has established strong links between stress and our moods and sense of well-being, not to mention overall mental and physical health. While certain types of stress during development can teach individuals to adapt to life’s many challenges, excessive and prolonged stress is shown to contribute to chronic disease and a range of psychological disorders. According to the American Psychological Association, which conducts an annual survey monitoring stress, the COVID pandemic has had an unprecedented impact on stress levels across all demographics, particularly among young people.

The pandemic is just one of many factors driving demand for new approaches to stress research. Thankfully, researchers now have access to a myriad of technologies to support any experiment design. In a recent webinar on techniques for measuring physiological changes related to stress, BIOPAC head of European Sales, Alex Dimov, outlined a range of stress-connected physiological responses that can be measured in the lab with available technologies. These include heart rate, heart rate variability (HRV), trans-radial-ulnar electrical bioimpedance velocimetry (TREV), cardiac output, blood pressure, EDA, pulse, respiration, EMG, EEG, and functional near infrared spectroscopy (fNIRS).

One of the challenges of measuring stress in a natural environment is finding the right data gathering tools to support the mobility of test participants. A 2019 study tackled this by examining a variety of techniques for measuring stress in day-to-day life using mobile phones and wearable sensors. Settings included the lab, automobile, office, school, and other daily life locations. Along with consumer products like smartphones, smartwatches, and fitness bands, researchers also employed wearable sensors, including the BioNomadix wireless PPG transmitter to measure blood volume pulse (BVP) using a photo plethysmogram (PPG) signal. Researchers found that the BIOPAC device provided a high level of accuracy (80%) when employed outside of the traditional lab environment.

Smelling RosesStress research technologies are not limited to data gathering. Stimulus presentation tools offer researchers options for both inducing and alleviating stress in the lab. A 2019 University of Wisconsin study investigating the role scents can play in reducing stress utilized EDA, heart rate, and respiratory rate to measure how rose scent reduced anxiety among students taking a difficult test. Respiratory rate was measured using a BIOPAC respiratory transducer while EDA data was collected with an EDA finger transducer. Data was collected and analyzed via the Biopac Student Lab System. While rose scent was delivered using scented lotion, it should be noted that BIOPAC offers a new modular scent delivery system that is capable of delivering up to eight scents per experiment, with more than 120 scent options.

With so many data gathering tools available, planning your next experiment should not leave you feeling on edge. Let your BIOPAC sales rep help you take the stress out of stress research.


BIOPAC offers a wide array of wired and wireless equipment that can be used in your research. To find more information on solutions for recording and analyzing signals such as ECG, heart rate, respiration, and more using any platforms mentioned in this blog post, you can visit the individual application pages on the BIOPAC website.

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