Noise Proof: Attack Artifacts Before They Ruin Data

After investing months of time, funds, and personal effort into your electrodermal activity (EDA) study, you begin combing through your data recordings when something catches your eye. Several of the recorded signals don’t look quite right. Rather than a narrow baseline in the signal graph, the baseline is thick and fuzzy, a sure sign of signal noise. To make matters worse, the recordings are broken by random peaks, artifacts unrelated to the EDA signal you sought to record. Noise and artifacts can corrupt research data, often rendering it useless. While it may be possible in some cases to clean up and salvage recorded data, such results often mean going back to square one and starting over.

The best option is to avoid bad data from the beginning. Several best practices can save researchers untold effort and expense by avoiding the pitfalls that can result in noise and artifacts. Noise and artifacts can be defined as unwanted changes to a signal caused by sources other than the target signal during recording. Noise and artifacts can originate from a range of sources—environmental (interference from other electromagnetic sources) or biological (signal changes from muscle activity in a participant), to name a few. The key is not to wait until all your data is recorded to tackle signal problems. Careful planning and testing procedures when designing and experimenting can avoid later headaches.

Most artifacts are caused by some form of electrical impedance interfering with a clean connection between the participant and the electrodes linking the data-gathering equipment. Sources could include dead skin or hair at the connection sight, dirty or improperly prepared electrodes, or electrodes that have come loose due to movement.

Using an impedance detector can help identify problematic signals before they corrupt data. An example of this can be found in a 2020 study at Ghent University in Belgium that examined how expert and novice map readers managed their cognitive loads using electroencephalogram (EEG) and eye tracking data. Researchers used a BIOPAC EL-CHECK impedance detector to ensure a good skin-electrode interface before recording data with AcqKnowledge software. This allowed the research team to keep the whole EEG circuit (ground, active EEG, and reference electrodes) at less than the recommended 10 K ohms (Herman, 2015; Teplan, 2002) to prevent unwanted signal noise.

noise checkStrategies for avoiding artifacts can vary depending on the type of signal being recorded. Some signals, such as EEG, may require light abrading of the skin at the point of electrode attachment for optimal conductance, while other signals, like EDA, do not require skin abrasion. Proper site preparation is recommended for most signals, skin should be clean and free of obstructions, such as excess hair. When gel is to be applied, make sure it is the correct type for the electrodes being used. Finally, the preparation of study participants can be critical to successfully recording clean data. In general, participants should be in an environment that is free of distractions and stimuli originating outside the study (sudden noises, distracting conversations, etc.).

For detailed information on how to proactively prevent signal noise and artifacts, check our AcqKnowledge Bootcamp webinar in which we discuss how to set up experiments to ensure quality data gathering. You can also consult our forthcoming guide with detailed instructions for the collection of optimal data.  Don’t wait until it’s too late; eliminating signal noise should be the first step in setting up a successful study.


Do you need help eliminating artifacts and signal noise in your recorded research data? BIOPAC’s regional sales representatives are ready to assist with tools and strategies to ensure you get only the best data possible. Contact us today!

Five Steps to a Better Physiology Lab – Preparation

Integrating new hardware and software into the Physiology lab curriculum…key points from instructors who have created successful labs that will greatly increase the chances of a rewarding lab experience for both students and instructor.

by William McMullen, BIOPAC Systems, Inc.

Integrating new hardware and software into the Physiology lab curriculum can be intimidating. Despite the best of early-summer intentions, in fall Instructors often find themselves apprehensively staring at a box full of unfamiliar equipment a few short days before the first lab. Luckily, the Biopac Student Lab excels in this situation, as it is easy to use and includes all materials necessary for students to prepare, record and analyze their data. This means that, in a crunch, if all one did was install the software and verify the hardware connections, most students should obtain meaningful data. Beyond the default setup, there are multiple customization options as described in the user support documentation. There are, however, some key points we have learned from instructors who have created successful labs that will greatly increase the chances of a rewarding lab experience for both students and instructor.

1. Organize the lab such that each student participates equally and each obtains recorded data from their bodies.
Students are more motivated when working with their own data. The Biopac Student Lab requires a minimum of three students for most lessons; a Recorder, a Director and a Subject. At the end of each lesson recordings, the students should rotate through these rolls and rerun the lesson until each student has recorded their own data. In order to provide enough recording time in a standard 2 hour lab period, the number of students per lab station should be four to five.

2. Setup basic Lesson Preferences before the first lab to minimize confusion.
Run each lesson* that will be used and setup the following:

  • If using lessons L02, L07-L09 or L12-L17, different models of transducers may be used. Check that the image and transducer descriptions in the lesson guide match the hardware used. If changes are needed, choose File > Lesson Preferences and select the desired transducer.
  • Disable undesired recording tasks by choosing File > Lesson Preferences > Lessons Recordings. If none of the recording tasks are desired (if using a custom procedure), disable all and only generic information will be displayed in the lesson guide.

*Note: If using the optional Curriculum Management System (available in BSL 4.1), only changes to the Host computer are required and all other computer/lab stations will be automatically updated. If not using this option, the changes must be made to each computer.

3. For Lessons L12 and L13 (Pulmonary Function), greatly reduce calibration time by matching the hardware with the software.
Label the lab station computer, the MP device and the Airflow Transducer (SS11LA/L) and make sure they are always matched. Run lesson 12 and check File > Preferences > Calibration Syringe Size to make sure it matches the syringe that will be used. Choose File > Preferences > Calibration Syringe Values and then select “Set once and use stored values”. Carefully run through the calibration using the syringe. From then on, when the students run the lesson, they will only need to calibrate for zero baseline (no syringe) which is easy and only takes a few seconds.

4. Students should have a basic understanding of the software before the first lab.
All Biopac Student Lab lessons use the same basic software tools and if students have a basic understanding of these tools in advance, they will experience greater success obtaining good data and having a positive lab experience. Students should be instructed to download the free Biopac Analysis software onto their personal computers.

This software not only provides the ability to analyze the data recorded in the lab but also allows students to prepare for a lab by studying the included tutorial videos and sample data. Some instructors give a test prior to the first lab to make sure students reviewed the BSL Tutorial Video.

5. Demonstrate proper electrode preparation and explain ways to minimize motion artifact. 
For lessons that use electrodes, good electrode contact and minimal EMG/motion artifact are keys to obtaining good data. Although this is explained in the procedures, it cannot be emphasized enough. Demonstrate the cleaning of the skin (with soap or alcohol) to remove dirt and oil and the abrading around the electrode site to remove the dead epidermal layers that impede electrical flow. Always place a drop of electrode gel on the electrode and wait at least 5 minutes after electrode placement before beginning a recording. To minimize EMG artifact, the student should be as relaxed and motionless as possible (unless procedure calls for movement). Talking, blinking (during EEG or EOG recording), laughing or deep breathing will add unwanted EMG artifact to the data.

These easily implemented steps will greatly assist students in obtaining good data from the very first lesson, leading to a positive lab experience and ultimately gaining a better understanding of Physiology.

 


BIOPAC Systems, Inc. provides life science researchers and educators with data acquisition and analysis systems that inspire people and enable greater discovery about life. BIOPAC lets you measure physiology anywhere, with innovative, compatible wireless, wearable solutions that can be used by anyone for meaningful discovery. We make high-quality scientific tools for physiology measurement and interpretation with superior compatibility and world-class customer service and support. Visit us at www.biopac.com.

error: Content is protected !!